Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENFIGSHMIYTYENGWEYEIYIKNDHTIDYRIHSGMVAGRWVRDQEVNIVKLTEGVYKVSWTEPTGTDVSLNFMPNEKRMHGIIFFPKWVHEHPEITVCYQNDHIDLMKESREKYETYPKYVVPEFAEITFLKNEGVDNEEVISKAPYEGMTDDIRAGRL 2P8G Chain:A ((3-162)) -ENFIGSHMIYTYENGWEYEIYIKNDHTIDYRIHSGMVAGRWVRDQEVNIVKLTEGVYKVSWTEPTGTDVSLNFMPNEKRMHGIIFFPKWVHEHPEITVCYQNDHIDLMKESREKYETYPKYVVPEFAEITFLKNEGVDNEEVISYAPYEGMTDDIRAGRL

General information: TITO was launched using: RESULT: Template: 2P8G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 792 -68766 -86.83 -429.78 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.99 3D Compatibility (PKB) : -86.83 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.594

(partial model without unconserved sides chains): PDB file : Tito_2P8G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P8G-query.scw PDB file : Tito_Scwrl_2P8G.pdb: