Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGELANYKRLEKVGEGTYGVVYKALDLRPGQGQRVVALKKIRLESEDEGVPSTAIREISLLKELKDDNIVRLYDIVHSDAHKLYLVFEFLDLDLKRYME-----GIPKDQPLGADIVKKFMMQLCKGIAYCHSHRILHRDLKPQNLLINKDGNLKLGDFGLARAFGVPLRAYTHEIVTLWYRAPEVLLGGKQYSTGVDTWSIGCIFAEMCNRKPIFSGDSEIDQIFKIFRVLGTPNEAIWPDIVYLPDFKPSFPQWRRKDLSQVVPSLDPRGIDLLDKLLAYDPINRISARRAAIHPYFQES 3EZV Chain:A ((3-290)) ----MENFQKVEKIGEGTYGVVYKARNKLTGE---VVALKKIR-------VPSTAIREISLLKELNHPNIVKLLDVIHTE-NKLYLVFEFLHQDLKKFMDASALTGIP------LPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGL--------------VVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQD-

General information: TITO was launched using: RESULT: Template: 3EZV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -170949 for 2032 contacts (-84.1/contact) + 2D Compatibility (PS) -27577 + (NN) -8725 + (LL) 1572 1D Compatibility (HY) -36000 + (ID) 8450 Total energy: -250129.0 ( -123.09 by residue) QMean score : 0.480

(partial model without unconserved sides chains): PDB file : Tito_3EZV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EZV-query.scw PDB file : Tito_Scwrl_3EZV.pdb: