Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDLTYIPEDLSSCPKFVNKILSSHQPLFSCPGDNVFGYDWSHDYPLFGNLVIMVSISHFKQLHSPTNFLILSMATTDFLLGFVIMPYSIMRSVESCWYFGDGFCKFHTSFDMMLRLTSIFHLCSIAIDRFYAVCYPLHYTTKMTNSTIKQLLAFCWSVPALFSFGLVL----SEADVSGMQSYKILVACFNFCALTFNKFWGTILFTTCFFTPGSIMVGIYGKIFIVSKQHARVISHVPENTKGAVKKHLSKKKDRKAAKTLGIVMGVFLACWLPCFLAVLIDPYLDYSTPILILDLLVWLRYFNSTCNPLIHGFFNPWFQKAFKYIVSGKIFSSHSETANLFPEAH 2YCW Chain:A ((28-298)) -----------------------------------------------GNVLVIAAIGSTQRLQTLTNLFITSLACADLVVGLLVVPFGATLVVRGTWLWGSFLCELWTSLDVLCVTASIETLCVIAIDRYLAITSPFRYQSLMTRARAKVIICTVWAISALVSFLPIMMHWWRDEDPQALKCYQD-PGCCDFVT---NRAYAIASSIISFYIPLLIMIFVALRVYREAKEQIRKID------RASKRKRVMLMREHKALKTLGIIMGVFTLCWLPFFLVNIVNVFNRDLVPDWLFVAFNWLGYANSAMNPIIYC-RSPDFRKAFKRLLAF-----------------

General information: TITO was launched using: RESULT: Template: 2YCW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -221458 for 2005 contacts (-110.5/contact) + 2D Compatibility (PS) -26563 + (NN) 3343 + (LL) 3356 1D Compatibility (HY) -23600 + (ID) 4200 Total energy: -269122.0 ( -134.23 by residue) QMean score : 0.271

(partial model without unconserved sides chains): PDB file : Tito_2YCW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YCW-query.scw PDB file : Tito_Scwrl_2YCW.pdb: