Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLSFSDTYRLNNGIEMPRHGFGVYKLTDEARMRTALETAVDVGYRLFDTASFYHNEKELGDFFASSGLKRDEFFVTTKMWNTEQGYDETLRAFEKSQKKLQLDQIDLYLVHWPKQDTFFDTWRAVEKLYDEGLVRAIGVSNFEAHHLDRLRTSANVLPVVDQLETHPHFPNHLLHRYLEELHIVHQAWSPLGRG---GVLQEQILIDLAEKHGKSPAQIVLRWHLQNNISIIPKSETPSRIRENAAIYDFELSEADMRQVERLNTGERVSHAPDVMYVRSEI 4GIE Chain:A ((14-289)) ------NCVTLHNSVRMPQLGLGVWRAQDGAETANAVRWAIEAGYRHIDTAYIYSNERGVGQGIRESGVPREEVWVTTKVWNSDQGYEKTLAAFERSRELLGLEYIDLYLIHWPGKKKFVDTWKALEKLYEEKKVRAIGVSNFEPHHLTELFKSCKIRPMVNQVELHPLFQQRTLREFCKQHNIAITAWSPLGSGEEAGILKNHVLGEIAKKHNKSPAQVVIRWDIQHGIVTIPKSTNKGRIQENFNVWDFKLTEEEMRQIDELNEDKRIGADPDNFFPGGE-

General information: TITO was launched using: RESULT: Template: 4GIE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -111499 for 2365 contacts (-47.1/contact) + 2D Compatibility (PS) -29832 + (NN) -14475 + (LL) 716 1D Compatibility (HY) -25600 + (ID) 6400 Total energy: -187090.0 ( -79.11 by residue) QMean score : 0.540

(partial model without unconserved sides chains): PDB file : Tito_4GIE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GIE-query.scw PDB file : Tito_Scwrl_4GIE.pdb: