Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHVKALAIKGIMTIIVLYLVLGLGFGFTFGDTLLMTIVLGVISYLLGDLYVLPKWNNMIATLADFGLAFLVIWLMGMPLSMGMTGGEVALAALFGAIVMAIGEYCFHFYMMSKEIGKKHYLETRTDS 3W9J Chain:A ((341-416)) ---VKTLGEAILLVFLVMYLFLQ-----NFRATLIPTIAVPVV--LLGTFGVL----------AAFGFSINTLTMFGMVLAIGL--------------------------------------------

General information: TITO was launched using: RESULT: Template: 3W9J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 120 -33994 -283.28 -531.15 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -283.28 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.144

(partial model without unconserved sides chains): PDB file : Tito_3W9J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3W9J-query.scw PDB file : Tito_Scwrl_3W9J.pdb: