Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVVRATSADVDLMARLLRAEAEGEGKQGMLLVGNVGINRLRANCSDFKGLRTIRQMIYQPHAFEAVTHGYFYQRARDSERALARRSINGERRW-PAKFSLWYFRPQGDCPAQWYNQPFVARFKSHCFYQPTAETCENVYNTF 4FET Chain:B ((109-220)) ---------DIQMMANAVYGESRGEPYLGQVAVAAVILNRVTS--ASFP--NTVSGVIFEPRAFTAVADGQIYLTPNETAKKAVLDAING---WDPTGNALYYFNPDTATSKWIWTRPQIKKIGKHIF---------------

General information: TITO was launched using: RESULT: Template: 4FET.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 519 -54649 -105.30 -492.33 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -105.30 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.244

(partial model without unconserved sides chains): PDB file : Tito_4FET.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FET-query.scw PDB file : Tito_Scwrl_4FET.pdb: