Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVRYQIEQLGDSAMMIRFGEEINEQVNGIVHAAAAYIEEQPFPGFIECIPAFTSLTVFYDMYEVYKHLPQGISSPFESVKRDVEERLAEIA--EDYEVNRRIVEIPVCYGGEFGPDLEEVAKINQLSPEEVIDIHTNGEYVVYMLGFAPGFPFLGGMSKRIAAPRKSSPRPSIPAGSVGIAGLQTGVYPISTPGGWQLIGKTPLALFRPQENPPTLLRAGDIVKFVRISEKDYHAYKEESN 2PHC Chain:B ((3-216)) ------IKPAGDSAFLISFGDEISEEINDRVHSLAKAIEKESPEWLVELVPAYSSLLVIYD--------------PLKASYEEVESYLKRISAREVERIKGKTIEIPVAYGGEFGPDIEFVAQYNGLSVDDVIEIHSKPLYRVYFLGFLPGFAYLGGMDERIATPRLEKPRLKVPAGSVGIAGKQTGWYAIESPGGWRIIGRIPLRTFNPGKVPPSIVLPGDYVKFVPIDEKEF--------

General information: TITO was launched using: RESULT: Template: 2PHC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1037 -134805 -129.99 -635.87 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -129.99 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_2PHC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PHC-query.scw PDB file : Tito_Scwrl_2PHC.pdb: