Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MARKEWKVLLIEDDPMVQEVNKDFITTVKGVTVCATAGNGEEGMKLIKEEQPDLVILDVYMPKKDGIKTLQEIRKQKLEVDVIVVSAAKDKETISLMLQNGAVDYILKPFKLERMRQALEKYKQYKQKIEANDTLSQEQLDAILNIPQQAVQDLPKGLNHFTMNEVTAFLKQQTASLSAEEVAKALGIARVTARRYLDYLEKTGIIKLDVQYGGVGRPVNRYVLKG 4IGA Chain:A ((7-121)) ------RVLIVDDAAFMRMMLKDIITKA-GYEVAGEATNGREAVEKYKELKPDIVTMDITMPEMNGIDAIKEIMKIDPNAKIIVCSAMGQQAMVIEAIKAGAKDFIVKPFQPSRVVEALNKV--------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4IGA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 616 -76893 -124.83 -668.63 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -124.83 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.547

(partial model without unconserved sides chains): PDB file : Tito_4IGA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IGA-query.scw PDB file : Tito_Scwrl_4IGA.pdb: