Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAARWHNQKDIRIEHIEEPKTEPGKVKIKVKWCGICGSDLHEYLGGPIFIPVDKPHPLTNETAPVTMGHEFSGEVVEVGEGVENYKVGDRVVVEPIFAT----HGHQGAYNLDEQMGFLGLAGGGGGFSEYVSVDEELLFKLPDELSYEQGALVEPSAVALYAVRSSKLKAGDKAAVFGCGPIGLLVIEALKAAGATDIYAVELSPERQQKAEELGAI-IVDPSKTDDVVAEIAERTG---GGVDVAFEVTGVPVVLRQAIQSTTIAGETVIVSIWEKG--AEIHPNDIVIKERTVKGIIGYRDIFPAVLSLMKEGYFSADKLVTKKIVLD---DLIEEGFGALIKEKSQVKILVRPN 4EJ6 Chain:A ((25-363)) -KAVRLESVGNISVRNVGIPEPGPDDLLVKVEACGICGTDRHLL------------HGEFPSTPPVTLGHEFCGIVVEAGSAVRDIAPGARITGDPNISCGRCPQCQAGRVNLCRNLRAIGIHRDGG-FAEYVLVPRKQAFEIPLTLDPVHGAFCEPLACCLHGVDLSGIKAGSTVAILGGGVIGLLTVQLARLAGATTVILSTRQATKRRLAEEVGATATVDPS-AGDVVEAIAGPVGLVPGGVDVVIECAGVAETVKQSTRLAKAGGTVVILGVLPQGEKVEIEPFDILFRELRVLGSFINPFVHRRAADLVATGAIEIDRMISRRISLDEAPDVISNPAAA-----GEVKVLVIPS

General information: TITO was launched using: RESULT: Template: 4EJ6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2007 -226585 -112.90 -695.05 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -112.90 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_4EJ6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EJ6-query.scw PDB file : Tito_Scwrl_4EJ6.pdb: