Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLQYRIIVDGRVQGVGFRYFVQMEADKRKLAGWVKNRDDGRVEILAEGPENALQSFVEAVKNGSPFSKVTDISVTESRSLEGHHRFSIVYS 2HLT Chain:A ((1-91)) MLQYRIIVDGRVQGVGFRYFVQMEADKRKLAGWVKNRDDGRVEILAEGPENALQSFVEAVKNGSPFSKVTDISVTESRSLEGHHRFSIVYS

General information: TITO was launched using: RESULT: Template: 2HLT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 427 -42202 -98.83 -463.76 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 1.00 3D Compatibility (PKB) : -98.83 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.571

(partial model without unconserved sides chains): PDB file : Tito_2HLT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HLT-query.scw PDB file : Tito_Scwrl_2HLT.pdb: