Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENQEVVLSIDAIQEPEQIKFNMSLKNQSERAIEFQFSTGQKFELVVYDSEHKERYRYSKEKMFTQAFQNLTLESGETYDFSDVWKEVPEPGTYEVKVTFKGRAENLKQVQAVQQFEVK 3OLO Chain:A ((7-89)) -------------QSELEFKFAHYLINN---AVEASFCLGDNWQFLYVNDATCRMTEYSREQLLSMNLQDIDVD----FALHD-WEEIRQKNNYTFKTRYRSQS---------------

General information: TITO was launched using: RESULT: Template: 3OLO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 240 -16996 -70.81 -204.77 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -70.81 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.372

(partial model without unconserved sides chains): PDB file : Tito_3OLO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OLO-query.scw PDB file : Tito_Scwrl_3OLO.pdb: