Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIPLHENLAGKTAVITGGSGVLCSAMARELARHGMKVAILNRTAEKGQAVVKEITAAGGTACAVAADVLDRMSLERAKEDILGQFGAVDLLINGAGGNHPDAITDVETYEEAGEGQSFFDMDERGFLTVFSTNFTGAFLASQVFGKELLKADSPAIINLSSMSAYSPMTKVPAYSAAKASINNFTMWMAVHFAETGLRVNAIAPGFFLTKQNHDLLINQDGTFTSRSHKIIAGTPMKRFGKPEDLLGTLLWLADESYSGFVTGITVPVDGGFMAYSGV 4J2H Chain:A ((9-255)) ------DLSGRRALVTGASRGIGQSIAVALAEAGAHVAVTARTVEGLAETRALIEKTGRRAVALAQDVRDVEACASVTRAAAEGLGGLDILVNNAG------------FENV---RPSFDVDEALWDTIVSTNLKGAFFCAQAAGRIMADANGGAIVNLCSLTSYVGIPTAVPYGASKSGLLGVTRALATEWAAHNIRVNAIAPGYFRTAMTAGFYEDED--WQSRMLEKI---PQRRFGKESDIGGVAVFLCSDA-AAYITGHCIPADGGYLA----

General information: TITO was launched using: RESULT: Template: 4J2H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1405 -150725 -107.28 -610.22 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -107.28 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_4J2H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J2H-query.scw PDB file : Tito_Scwrl_4J2H.pdb: