Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWRNASMNHSKRNDANDFDSMDEWLRQFFEDPFAWYDETLPIDLYETSQQYIIEADLTFLQPTQVTVTLSG-CEFILTVKSSGQ------------TFEKQMMLPFYF-NDKNIQVECENQILTVAVNKETEDGSSFSLQFPLS 4YL9 Chain:A ((12-113)) -------------------KLDELSREFYESVIP------PIDMYEEGGELVVVADLAGFNKDKISVRLSAQNELIINAEREIQYIGTKYATQRPLKIHKVIRLPVKVKRDSQVTAKYENGVLTIRI-----------------

General information: TITO was launched using: RESULT: Template: 4YL9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 250 -14044 -56.17 -159.59 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -56.17 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_4YL9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YL9-query.scw PDB file : Tito_Scwrl_4YL9.pdb: