Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKAIQSSMWREFGLMCTVGIGDNMLLSKLALDLESKKTKSGIARWRYEDVPNRLWKVRPLSKMWGIGGRMERNLNRMGISTVGQLAKFPLELLEKKFGIMGNQLYYHAHGIDLSEIGAPLMQGQISFGKSQILFRDYTKREEIKAVLLEICEEVARRARTYNKVGRTISLGIGYSKDELGGGFHRSKTIDFPTNITMDIYKCCLMFFNKFYSGKKQ 4R8U Chain:A ((122-273)) IAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNGQFVIT---PAEVPAFLQTL-PLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFGRILWERSQGIDERDVNSERLRK--SVGVERTMAEDIHHWSECEAIIERLYPELERR-----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4R8U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 442 -55307 -125.13 -363.86 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -125.13 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.402

(partial model without unconserved sides chains): PDB file : Tito_4R8U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R8U-query.scw PDB file : Tito_Scwrl_4R8U.pdb: