Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFHDDKKNELQKKEEIITEAID---------------TLFQSSAFGNLING-------FQNLINSSLKDVQTTI-----HVRERDTGL--YIDITIPATFRDGEIVVDVKSRYLHVTLQEKQKHQNEATFTSMTRTVQLPYEVRQEDMETSWNEQTMTLFFPKNKHE 2UX0 Chain:F ((11-100)) ------RKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVEGMDFHKFYFENLLSKNSKPIHTTILNPHVHVIGEDAACIAYIRLT------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2UX0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 135 -5903 -43.72 -96.76 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain F : 0.66 3D Compatibility (PKB) : -43.72 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.305

(partial model without unconserved sides chains): PDB file : Tito_2UX0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2UX0-query.scw PDB file : Tito_Scwrl_2UX0.pdb: