Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEKIIKQKKEEVKTLVLPVEQPFEKRSFKEALASPNRFIGLIAEVKKASPSKGLIKEDFVPVQIAKDYEAAKADAISVLTDTPFFQGENSYLSDVKRAVSIPVLRKDF-IDSLQVEESRRIGADAILLIGEVLDPLHLHELYLEAGEKGMDVLVEVHDASTLEQILKVFTPDILGVNNRNLKTFETSVKQTEQIASLVPKESLLVSESGIGSLEHLTFVNEHGARAVLIGESLMRQTSQRKAIHALFRE 1I4N Chain:A ((31-241)) ----------------------------FLEVLSGKER-VKIIAEFKKASPSAGDINADASLEDFIRMYDEL-ADAISILTEKHYFKGDPAFVRAARNLTCRPILAKDFYIDTVQVKLASSVGADAILIIARILTAEQIKEIYEAAEELGMDSLVEVHSREDLEKVFSVIRPKIIGINTRDLDTFEIKKNVLWELLPLVPDDTVVVAESGIKDPRELKDLR-GKVNAVLVGTSIMKAENPRR--------

General information: TITO was launched using: RESULT: Template: 1I4N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1037 -49253 -47.50 -234.54 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -47.50 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.468

(partial model without unconserved sides chains): PDB file : Tito_1I4N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1I4N-query.scw PDB file : Tito_Scwrl_1I4N.pdb: