Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMNIVLARIDDRFIHGQILTRWIKVHAADRIIVVSDDIAQDEMRKTLILSVAPSNVKASAVSVSKMAKAFHSPRYEGVTAMLLFENPSDIVSLIEAGVPIKTVNVGGMRFENHRRQITKSVSVTEQDIKAFETLSDKGVKLELRQLPSDASEDFVQILRNVTK 1VSQ Chain:C ((4-165)) -MVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTAHVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQVNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK

General information: TITO was launched using: RESULT: Template: 1VSQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 753 -121094 -160.82 -747.49 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.85 3D Compatibility (PKB) : -160.82 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.568

(partial model without unconserved sides chains): PDB file : Tito_1VSQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VSQ-query.scw PDB file : Tito_Scwrl_1VSQ.pdb: