Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIFSLALISLFAVILLAACGSKGSNGEASKESKKDTLAAIKDNDKIVFGVKTDTRLFGLKNPSSGEIEGFDIDIAKQIAKDILGDEKKAQFKEVTSKTRIPMLQNGDIDAIVATMTITEERKKEVDFSDVYFEAGQSLLVKKGS-KIKSVENLGKGSKVLAVKGSTSSQNIREKAPEAS--VLEFENYAEAFTALKSGQGDALTTDNAILYGMADENKN--YQLTGKPFTDEPYGIAVKKGQSALAKEINASLKKMKSDGRYDEIYKKWIKEDPAE 4ZV1 Chain:A ((23-232)) ----------------------------------------------------------FGFKD-ENGKLVGFDIDLAKAIAKK-LG--VKVEFKPMDFDGIIPALQSGKIDVVIAGMTITEERKKQVDFSDPYFEAGQAIVVKKGNDSIKSLEDL-KGKKVGVQLGSTSEQHVKKVAKDAGVKVKKFDNFSEAFQELKSGRVDAVVTDNAVALAYVKQNPNAGVKIVGETFSGEPYGIAVRKGNSELLEKINKALEEMKKDGTYDKIYEKWFG-----

General information: TITO was launched using: RESULT: Template: 4ZV1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 983 34658 35.26 169.06 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : 35.26 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.528

(partial model without unconserved sides chains): PDB file : Tito_4ZV1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZV1-query.scw PDB file : Tito_Scwrl_4ZV1.pdb: