Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVDLFDFELPERLIAQVPLEQRDASRLMVLDKHTGELTDSSFKHIISFFNEGDCLVLNNTRVLPARLFGTKEDTGAKVELLLLKQETGDKWETLAKPAKRVKKGTVVTFGDGRLKAICTEELEHGGRKMEFQY--DGIFYEVLESLGEMPLPPYIKEQLDDKERYQTVYSKEIGSAAAPTAGLHFTEEILQQLKDKGVQIEFITLHVGLGTFRPVSADEVEEHNMHAEFYQMSEETAAALNKVRENGGRIISVGTTSTRTLETIAGEHDGQFKASSGWTSIFIYPGYEFKAIDGMITNFHLPKSSLIMLVSALAGRENILRAYNHAVEEEYRFFSFGDAMLII 1VKY Chain:A ((16-347)) ---SEFDYELPPELIAQEPVEPRDASRLMVLHRKTQRIEHRIFREIIEYLEPGDLLVLNVSKVIPARLYARK----ASIEILLIERLEEGIWKCLVRPGQKVKKGTELVI-DEDLSAVCLGRGEDGTRILKFQPQDDRLIFEK----G--------------------------------TAGLHFTPELIEKLKKKGVQFAEVVLHV------------------HEEFYQVPKETVRKLRETRERGNRIVAVGTTTVRTLETIARLPEQE--EYVGKTDLFIYPPFEFKLVDALVTNFHLPRSTLLMLVAAFAGKDFVMEAYREAVKRRYRFFSFGDAMLIL

General information: TITO was launched using: RESULT: Template: 1VKY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1432 -92342 -64.48 -332.17 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -64.48 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_1VKY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VKY-query.scw PDB file : Tito_Scwrl_1VKY.pdb: