Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFTAYQDARSWIHGRLKFGVKPGLGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNERISVNGIPISDEEWTALVNQMKPHVEALDQTEYGQP----TEFEIMTACAFLYFAEFHKVDFVIFETGLGGRFDSTNVVEPLLTVITSIGHDHMNILGNTIEEIAGEKAGIIKEGIPIVTAVTQPEALQVIRHEAERHAAPFQSLHDACVIFNEEALPAGEQFSFKTEEKCYEDIRTSLIGTHQRQNAALSILAAEWLNKENIAHISDEALRSGLVKAAWPGRLELVQEHPPVYLDGAHNEEGVEKLAETMKQRFANSRISVVFSALKDKPYQNMIKRLETIAHAIHFASFDFPRASLAKDLYDASEIS--NKSWSEDPDDVIKFIESKKGSNEIVLITGSLYFISDIRKRLK 1FGS Chain:A ((3-423)) ----YTETVAYIHSFPRLA--GDHRRILTLLHALGNPQQQGRYIHVTGTNGKGSAANAIAHVLEASGLTVGLYTSPFIMRFNERIMIDHEPIPD---AALVNAVAFVRAALERLQQQQADFNVTEFEFITALAYWYFRQ-RQVDVAVIEVG-----DSTNVITPVVSVLTEVALD-------TITAIAKHKAGIIKRGIPVVTGNLVPDAAAVVAAKVATTGSQWLRFDRDFSVPKAKLHGWGQRFTYEDQDGRISDLEVPLVGDYQQRNMAIAIQTAKVYAKQTEWPLTPQNIRQGLAASHWPARLEKISDTPLIVIDGAHNPDGINGLITALKQLFSQP-ITVIAG------YAAMADRLTAAFSTVYLVPVPGTPR------------GRLKDSWQEALAASLNDV-----PDQPIVITGSLYLASAVRQTL-

General information: TITO was launched using: RESULT: Template: 1FGS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2122 -61600 -29.03 -160.83 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -29.03 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.568

(partial model without unconserved sides chains): PDB file : Tito_1FGS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FGS-query.scw PDB file : Tito_Scwrl_1FGS.pdb: