Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKIIAINAGSSSLKFQLFEMPSETVLTKGLVERIGIADSVFTIS-VNGEKNTEVTDIPDHAVAVKMLLNKLT--EFGIIKDLNEIDGIGHRVVHGGEKFSDSVLLTDETIKEIEDISELAPLHNPANIVGIKAFKEVLPNVPAVAVFDTAFHQTMPEQSYLYSLPYEYYEKFGIRKYGFHGTSHKYVTERAAELLGRPLKDLRLISCHLGNGASIAAVEGGKSIDTSMGFTPLAGVAMGTRSGNIDPALIPYIMEKTGQTADEVLNTLNKKSGLLGISGFSSDLRDIVEATKEGNERAETALEVFASRIHKYIGSYAARMSGVDAIIFTAGIGENSVEVRERVLRGLEFMGVYWDPALNNVRGEEAFISYPHSPVKVMIIPTDEEVMIARDVVRLAK 1TUU Chain:A ((2-391)) --KVLVINAGSSSLKYQLIDMTNESALAVGLCERIGIDNSIITQKKFDGKKLEKLTDLPTHKDALEEVVKALTDDEFGVIKDMGEINAVGHRVVHGGEKFTTSALYDEGVEKAIKDCFELAPLHNPPNMMGISACAEIMPGTPMVIVFDTAFHQTMPPYAYMYALPYDLYEKHGVRKYGFHGTSHKYVAERAALMLGKPAEETKIITCHLGNGSSITAVEGGKSVETSMGFTPLEGLAMGTRCGSIDPAIVPFLMEKEGLTTREIDTLMNKKSGVLGVSGLSNDFRDLDEAASKGNRKAELALEIFAYKVKKFIGEYSAVLNGADAVVFTAGIGENSASIRKRILTGLDGIGIKIDDEKNKIRGQEIDISTPDAKVRVFVIPTNEELAIARE------

General information: TITO was launched using: RESULT: Template: 1TUU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2209 -40390 -18.28 -104.37 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -18.28 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.603

(partial model without unconserved sides chains): PDB file : Tito_1TUU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TUU-query.scw PDB file : Tito_Scwrl_1TUU.pdb: