Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLNDILEFNKTFTEQREYEKYQTSKFPDK---KMAILSCMDTRLVELLPHAMNLRNGDVKIIKSAGALVTHPFGSIMRSILVAVYELNADEVCVIGHHDCGMSKISSKSMLEKIKARGIPEERIETIKYSGVDF-DQWFKSFDSVEASVKDSVDVIKHHPLFPENVPVHGLVIDPKTGKLDLIVNGYNN 3LAS Chain:A ((3-162)) MSYFDNFIKANQAYVDL-----HGTAHLPLKPKTRVAIVTCMDSRL-HVAP-ALGLALGDAHILRNAGGRVTD---DVIRSLVISEQQLGTSEIVVLHHTDCGAQTFTNAEFTEQLK------------RDLAVDAGDQDFLPFTDIEESVREDIALLKNSPLIPEDIIISGAIYDVDTGRV---------

General information: TITO was launched using: RESULT: Template: 3LAS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 601 -25056 -41.69 -160.61 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -41.69 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.656

(partial model without unconserved sides chains): PDB file : Tito_3LAS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LAS-query.scw PDB file : Tito_Scwrl_3LAS.pdb: