Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKILVTGAAGFIGSHLCEELLK-DKKHNVIGIDDFIGPTPFSLKLKNLKNLLPEKR---FTFIKENLLT-ADLASLLEGVDVIFHLAAIPGVRSSWGNHFHPYAAHNIQALQRLLEACREHSIQTFVFASTSSVYGEKQG--KVSENTSLSPLSPYGVTKLTGEKLCHVYKQSFGIPIVILRFFTVYGPRQRPDMAFHRLIKQ----HLQQKPLTIFGDGQQSRDFTYISDCVKG-ITAVLGKPHLIGETVNIGGAERASVLKVVSLIED-ISGRKATLH----FSDKIAGEPSNTWADISKAKQLLHYDPATSLKDGLTNEIAYLSSLYQGE 1SB9 Chain:A ((31-341)) ---LITGVAGFIGSNLLETLLKLDQK--VVGLDNF--ATGHQRNLDEVRSLVSEKQWSNFKFIQGDIRNLDDCNNACAGVDYVLHQAALGSVPRSINDPITSNAT-NIDGFLNMLIAARDAKVQSFTYAASSSTYGDHPGLPKV-EDTIGKPLSPYAVTKYVNELYADVFSRCYGFSTIGLRYFNVFGRRQDPNGAYAAVIPKWTSSMIQGDDVYINGDGETSRDFCYIENTVQANLLAATAGLDARNQVYNIAVGGRTSLNQLFFALRDGLAENGVSYHREPVYRDFREGDVRHSLADISKAAKLLGYAPKYDVSAGVA-------------

General information: TITO was launched using: RESULT: Template: 1SB9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1649 -22596 -13.70 -76.86 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -13.70 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.427

(partial model without unconserved sides chains): PDB file : Tito_1SB9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SB9-query.scw PDB file : Tito_Scwrl_1SB9.pdb: