Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEDKMDLYLQQ-GMYGPLETKPD-ERHLFLGSLRERVVLALTKGQVLRSKPYK--EAEHELKNSHNVTLL-------INGELQYQSYSS--YIQMASRYGVPFKI-VSDLQFHTPLGIVIAADIAVNRELIYIQDDIYNRSVLKS 4G3N Chain:A ((530-660)) ----KTDLYRSQK---GAGLKQDDIVAHFFVCSTHDLILFFTTQGRVYRAKAYDLPEASRTARGQHVANLLAFQPEERIAQVIQIRGYTDAPYLVLATRNGLVKKSKLTDFDSNRSGGIV-AVNLRDNDELV--------------

General information: TITO was launched using: RESULT: Template: 4G3N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 335 -9682 -28.90 -88.02 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -28.90 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.237

(partial model without unconserved sides chains): PDB file : Tito_4G3N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G3N-query.scw PDB file : Tito_Scwrl_4G3N.pdb: