Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTYKGYLIDLDGTMYNGTEKIEEACEFVRTLKDRGVPYLFVTNNSSRTPKQVADKLVSFDIPATEEQVFTTSMATAQHIAQQKKDASVYVIGEEGIRQAIEENGLTFGGENADFVVVGIDRSITYEKFAVGCLAIRNGARFISTNGDIAIPTERGLLPGNGSLTSVLTVSTGVQPVFIGKPESIIMEQAMRVLGTDVSETLMVGDNYATDIMAGINAGMDTLLVHTGVTKREHMTDDMEKPTHAIDSLTEWIPYI 4O8C Chain:A ((7-258)) MRKYNGYLIDLDGTMYRGTERIDAASGFIKELNRLHIPYLFVTNNSTRTPEQVADKLVSLDIPATPEQIFTSSMATANYVYDLDQNAMIYFIGEEGLYKALKEKGFSFADENADVVIVGLDREVTYEKLAVACLAVRNGAKLISTNGDLALPTERGFMPGNGAFTALISYSTQVKATFVGKPEPIIMEQALKVLGTNKNETIMVGDNYDTDILAGIRAGLDTLLVHTGVTTVEKLKEYKQQPTYSMKSLDDW----

General information: TITO was launched using: RESULT: Template: 4O8C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1387 -137752 -99.32 -546.63 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.92 3D Compatibility (PKB) : -99.32 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.635

(partial model without unconserved sides chains): PDB file : Tito_4O8C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4O8C-query.scw PDB file : Tito_Scwrl_4O8C.pdb: