Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIINKPSRVRPDGRGKVTGELKYMTDLSFPGMLYGKVLRSAYPHAEIVSVCTIKAEKMEGVQAVVTHKDVPGLNRFGI--VIPDQPVLCEDRVRYVGDAIAAVAAETEEIAEAALELIQVEYKELEVMDSPEKALRPNAQRLHED--GNILHRAFFSNGDVEEGFQASDTVFEETYELPRQMHTYMETEGGVAV--PEDDGGFTMYAGTQHGYKDRFQLARIFDIPEEKIRIVSSPMGGSFGGKDE-LNIQPYAALLALKSGRPVKIHQTRKESVRSGIKRHPMKITIKTGADHSGNLLAHDVKIVADTGAYATLGPAVLDFSVEHAAGPYRIPNIRTEGISVFTNNGVAGEFRGFGGNQITFALETHLDRLSGMLGIDPLELRRKNIRKPHDLGPLEHRIAPTDGAAQVLNAISKSPILKKTSRNCGYLQR--------GTGAAITMHGGGLGFGRMDA----AGGRLSLSSEGKITASFGFEECGQGILAAIEQIVMEELGCAAEDISIVIGDTAKVPKSGSSTASRGTSMVWHAIQRLKKPFLAQLKKRAAEWSGCSAENL-----IPGAAGLRDKNTKALVVTYKELAEKGPLAEETAFDFPT-------TPDPVVGGHFLYSFGAAAVEVEVDLLTGDVKLIDCEHAIAAGPVVSPQGYRGQIEGGAAMALGYTLMEEAKMTDGRYAAENLDHYLIPGIKDVPDMKLIAIEDLMKGDVYGPRGVGEIGTIAITPAIVKAVHDAVGCWINKLPISREELLEAIDRKGLKQWT 1RM6 Chain:A ((19-765)) -----------DGVEKVTGKAKYTADIAAPDALVGRILRSPHAHARILAIDTSAAEALEGVIAVCTGAETP--VPFGVLPIAENEYPLARDKVRYRGDPVAAVAAIDEVTAEKALALIKVDYEVLPAYMTPKAAMKAGAIALHDDKPNNILREVHAEFGDVAAAFAEADLIREKTYTFAEVNHVHMELNATLAEYDPVRDM-LTLNTTTQVPYYVHLKVAACLQMDSARIRVIKPFLGGGFGARTEALHFEIIAGLLARKAKGTVRLLQTREETFIAHRGRPWTEVKMKIGLKKDGKIAALALEATQAGGAYAGYGIITILYTGALMHGLYHIPAIKHDAWRVYTNTPPCGAMRGHGTVDTRAAFEALLTEMGEELGIDSLKIRQINMLP---------QIPYVTMYAQRVMSYGVPECLEKVKAASGWEERKGKLPKGRGLGIALSHFVSGTSTPKHWTGEPHATVNLKLDFDGGITLLTGAADIGQGSNTMASQVAAEVLGVRLSRIRVISADSALTPKDNGSYSSRVTFMVGNASISAAEELKGVLVKAAAKKLDAREEDIEVIDEMFMVSGSQDPGLSFQEVVKAAMVDSGTITVKGTYTCPTEFQGDKKIRGSAIGATMGFCYAAQVVEASVDEITGKVTAHKVWVAVDVGKALNPLAVEGQTQGGVWMGMGQALSEETVYDNGRMVHGNILDYRVPTIVESPDIEVIIVESMDPNGPFGAKEASEGMLAGFLPAIHEAVYEAVGVRATDFPLSPDRITELLDAK------

General information: TITO was launched using: RESULT: Template: 1RM6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4809 61218 12.73 85.50 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 12.73 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.464

(partial model without unconserved sides chains): PDB file : Tito_1RM6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RM6-query.scw PDB file : Tito_Scwrl_1RM6.pdb: