Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNENMSFKELYAIVRHRFVLILLITIGVTLIMGFVQFKVISPTYQASTQVLVHESDGEENSNLSDIQRNLQYSSTFQSIMKSTALMEEVKAELHLSESASSLKGKVVTSSENESEIINVAVQDHDPAKAAEIANTLVNKFEKEVDERMNVQGVHILSEAKASESPMIKPARLRNMVMAFGAAVMGGITLAFFLHFLDDTCKSARQLSERTGLPCLGSVPDVHKGRNRGIKHFGE 4Q6N Chain:A ((89-187)) --------------------------------------------------------GDMNRKFAALDKNDPEYPTIQEI-KSLIAKPSIDAVLHLHDGGGYYRPVYVDAMLNPKRWGNCFIIDQDEVKGAKFPNLLA--FANNTIESINAHLLHPIEE----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4Q6N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 205 -4496 -21.93 -45.41 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -21.93 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.188

(partial model without unconserved sides chains): PDB file : Tito_4Q6N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q6N-query.scw PDB file : Tito_Scwrl_4Q6N.pdb: