Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKPFVFITKPIPEEIEAFIGEH-CRYEVWQEDTLPSDVLFEKLKEAEGLLTSGTSGPSINRELLEHAPKLKVVSNQSVGYDNFDIEAMKERGVVGTHTPYTLDDTVADLAFSLILSSARRVAELDRFVRAGKW---GTVEEEALF-GIDVHHQTLGIIGMGRIGEQAARRAKFGFDMEVLYHNRHRKQETEDSIGVKYAELDTLLEQSDFILLITPLTDETYHMIGEREFKLMKNSAIFVNISRGKTVDEKALIRALQEGWIRGAGLDVYEKEPVTQDNPLLQLDNVTLLPHIGSATAKVRFNMCKQAAENMLAAIQGQTPKNLTREFQ 5AOW Chain:A ((1-321)) -MKPKVLITRAIPENGIELLREHFEVEVWEHEHEIPREVLLEKVKDVDALVTMLSE--KIDREVFDAAPRLRIVANYAVGYDNIDIEEATKRGIYVTNTPDVLTDATADLAWALLLAAARHVVKGDKFVRSGEWKRRGIAWHPKMFLGYDVYGKTIGIVGFGRIGQAIAKRAK-GFGMRILYTARSRKPEAEKELGAEFKPLEELLRESDFVVLAVPLTKETYHMINEERLRLMKPTAVLVNVARGKVVDTKALIRALKEGWIAAAGLDVFEEEP-YYDEELFALDNVVLTPHIGSATFGAREGMAELVAKNLIAFKNGEVPPTLV----

General information: TITO was launched using: RESULT: Template: 5AOW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1679 23896 14.23 75.62 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : 14.23 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.584

(partial model without unconserved sides chains): PDB file : Tito_5AOW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AOW-query.scw PDB file : Tito_Scwrl_5AOW.pdb: