Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAESLLIKDIAIVTENEVIKNGYVGINDGKISTVSTERPKEPYSKEIQAPADSVLLPGMIDIHIHGGYGADTMDASFSTLDIMSSRLPEEGTTSFLATTITQEHGNISQALVNAREWKAAEESSLLGAELLGIHLEGPFVSPKRAGAQPKEWIRP-SDVELFKKWQQEAGGLIKIVTLAPEEDQHFELIRHLKDESIIASMGHTDADSALLSDAAKAGASHMTHLYNAMSPFHHREPGVIGTALAHDGFVTELIADGIHSHPLAAKLAFLAKGSSKLILITDSMRAKGLKDGVYEFGGQSVTVRGRTALLSDGTLAGSILKMNEGARHMREFTNCSWTDIANITSENAAKQLGIFDRKGSVTVGKDADLVIVSSDCEVILTICRGNIAFISKEADQI 1O12 Chain:A ((29-376)) --------------------TGDVEIEEGKIVKVEKR----------ECIPRGVLMPGFVDPHIHGVVGADTMNCDFSEMEEF---LYSQGVTTFLATTVSTSLEKMKEILRKARDYILENPST----SLLGVHLEGPYISKEKKGAHSEKHIRPPSEREL-----SEIDSPAKMLTFAPEIESS-ELLLRLVKRDIVLSAGHSIATFEEFMKFYKEGVKRITHFPNGLKPLHHREIGITGAGLLLDDVKLELICDGVHLSREMVKLVYKVKKANGIVLVTDSISAAGLKDGTTTLGDLVVKVKDGVPRLEDGTLAGSTLFFSQAVKNFRKFTGCSITELAKVSSYNSCVELGLDDR-GRIAEGTRADLVLLDEDLNVVMTIKEGEVVFRSR-----

General information: TITO was launched using: RESULT: Template: 1O12.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2155 -40119 -18.62 -115.62 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -18.62 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_1O12.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O12-query.scw PDB file : Tito_Scwrl_1O12.pdb: