Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTASKFSVMFFMLLALCGCWDVKDIEQLSFARGLAIDETNDHQYKLTYQNLLPQSEDSQASGKPEFVNVTSHGKTILEAVSDVSIKDPPVYSDHLKVILLGEKLMRNQNVDQVLNHFIRDDELRRSSYLMAARGNAADVFTKGNPNQQQPMPSEKLIDLTTHSGYNGKIMIPLRIGRA-----SVYSQNGYSYLIQAVKNEKGKAKYDGAGIIKRGSNKLVG--FLSADETQTLSWVMGTIQGGVMPTTDKGH------PITFEIKKSKTKIKPVIENGKPVFHISVKTKGILTEDQNPNENSFSKSYLHRLENIFEKKLERDVKQVMDKLQHEYKTDPVFLSDHIRIQHPDYWNKVKGHWDEIFSETDFKYDISFKIINFGTVGK 1UYP Chain:A ((195-297)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------VRIGEKDILIYSITSTNSVLFSMGELK--EGKLNVEKRGLLDHGTDFYAAQTFFGTDRVVVIGWLQSWLRTGLYPTKREGWNGVMSLPRELYVENNELKVKPVDE-------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1UYP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 314 22595 71.96 251.06 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 71.96 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.096

(partial model without unconserved sides chains): PDB file : Tito_1UYP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UYP-query.scw PDB file : Tito_Scwrl_1UYP.pdb: