Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREELKGIFASVFGEKEGLRFFFAPGRVNLIGEHTDYNGGHVFPCALTMGTYAAVAERNDGLVRMYSDNFRNAGIKECSLDDIRYQKEDDWANYPKGVIYEFQQRGYAVPHGFDIVFSGNIPNGAGLSSSASIELLMGVVLQSYFHPEVDALELVKMAQHAENHFIGVNCGIMDQFAIGMGKKHHAMLLNCDTLDYEYSKLNVSGLALVIANTNKKRTLADSSYNTRRQECNDALLDLKKGLDIAALGDIKPSDFDAHSSLIQNETNRRRAKHAVYENHRAIKTAHMFKENNIDEIGQLMKESHLSLKDDYEVTCPELDELVFAAWDHEGVIGSRMTGAGFGGCTISIVKDEFVDDFIQKVGDRYQEKTGLRADFYVADIGEGARELKGE 2CZ9 Chain:A ((6-348)) -----------------------SPGRVNLIGEHTDYTYGYVMPMAINL--YTKIEAEKHGEVILYSEHFGEE--RKFSLNDLR--KENSWIDYVKGIFWVLKESDYEV-GGIKGRVSGNLPLGAGLSSSASFEVGILETLDKLYNLKLDSLSKVLLAKKAENEFVGVPCGILDQFAVVFGREGNVIFLDTHTLDYEYIPFP-KDVSILVFYTGVR----SSEYAERKHIAEESLKILGKG----SSKEVREGELSKLPPL-----HRKFFGYIVRENARVLEVRDALKEGNVEEVGKILTTAHWDLAKNYEVSCKELDFFVERALKL-GAYGARLTGAGFGGSAIALVDKEDAETIGEEILREYLKRFPWKARHFIVEPSDGV------

General information: TITO was launched using: RESULT: Template: 2CZ9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2004 -198617 -99.11 -585.89 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -99.11 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_2CZ9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CZ9-query.scw PDB file : Tito_Scwrl_2CZ9.pdb: