Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGYAERATTRRRQAGFTLIEVMVAIMLMAIVSLMAWRGLDSIARASAHLEDSTEQGAALLRALNQLERDIALHSAIREETGLPSGDEPIRAGDSLPPGLALKRLSEIPLRLDIVRASTEPGAPLQRVRWWRQGKVLYRAASPSGDRLPLPPLAERVA----VLDDVS--RFDIRAWVPGKGWTRLPARSKVRASGLEISLARVTRNGVERYRRVVALQ 4HHJ Chain:A ((396-435)) -----------------------------------------------------------------------------------------------------------------------------------------------SGDDCVVKPIDDRFANALLALNDMGKVRKDIPQWQPSKGWHDWQQVPFCSHHFHELIMKDGRKLVVPCRPQDELIG

General information: TITO was launched using: RESULT: Template: 4HHJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -1230 for 113 contacts (-10.9/contact) + 2D Compatibility (PS) -3494 + (NN) -2244 + (LL) 11220 1D Compatibility (HY) -2400 + (ID) 800 Total energy: 1052.0 ( 9.31 by residue) QMean score : 0.144

(partial model without unconserved sides chains): PDB file : Tito_4HHJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HHJ-query.scw PDB file : Tito_Scwrl_4HHJ.pdb: