Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLIGYFAYKRTSNLTDYMLGGRSLGPAVTALSAGAADMSGWLLMGLPGAMFSTGLSGAWIVIGLCLGAWANWLYVAPRLRTYTEKAGNSITIPGFLENRFGDQTKLLRLFSGIVILVFFTFYVSSGMVSGGVLFNSILGMDYHTGLWIVTGVVVAYTLFGGFLAVSWTDFVQGIIMFAALILVPIVTFFHTGGAGDTVAEIRSV--DPDMFNIFKGTSVLGIISLFAWGLGYFGQPHIIVRFMAITSVKEIKRARRIGMGWMILSAVGAVLTGLGGIAYYHQRGMTLKDPETIFIQLGNILFHPIITGFLISAILAAIMSTISSQLLVTSSSLVEDLYKSMFRRSASDKELVFLGRLAVLAVSIVALVLAWEKNNTILGLVSYAWAGFGASFGPVVLLSLFWKRMTKWGALAGMIVGAATVIIWANAGLSDFLYEMIPGFAASLLSVFFVSILTQAPSQAVTDQFNDYQDTMSQ------ 3SAF Chain:A ((1-410)) SIIRPQLKFREKIDNSN-----TPFLPKIFIKPNAQKPLP----QALSKERRQDMFAHPYQYELNHFTPADAVLQKPQPQLYRPIEETPCHFISS--LDELVELNEKLLNCQEFAVNLEHHSYRS----------------------------------FLGLTCLMQISTRTEDFIIDTLEL------------RSDMYILNESLTDPAIVKVFHGA-----DSDIEWLQKDFG-LYVVNMFDTHQAARLLNLGRH-SLDHLLKLYCNVDSNKQYQLADWRIRPLP-----EEMLSYARDDTHY----------LLYIYDKMRLEMWERGNG-QPVQLQVVWQRSRDI----CLKKFIKPIFTDESYLELYRKQKKHLN----------------TQQLTAFQLLFAWRDKTARREDESYGYVLPNHMMLKIAEELPKEPQGIIACCNPVPPLVRQQINEMHLLIQQAREMPLLKSEVAA

General information: TITO was launched using: RESULT: Template: 3SAF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1795 -245095 -136.54 -657.09 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -136.54 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.254

(partial model without unconserved sides chains): PDB file : Tito_3SAF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SAF-query.scw PDB file : Tito_Scwrl_3SAF.pdb: