Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFRLLVLMGFTFFFYHLHASGNLTKYINMKYAYLSFIAIFLLAILTAVQAYLFIKSPEKSGHHHDHDCGCGHDHEHDHEQNKPFYQRYLIYVVFLFPLVSGIFFPIATLDSSIVKTKGFSFKAMESGDHYSQTQYLRPDASLYYAQDSYDKQMKQLFNKYSSKKEISLTDDDFLKGMETIYNYPGEFLGRTIEFHGFAYKGNAIN--KNQLFVLRFGIIHCIADS-----GVYGMLVE--FP--K-DMDIKDDEWIHIKGTLASEYYQPFKSTLPVVKVTDWNTIKKPDDPYVYRGF 3FHW Chain:A ((3-100)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLELSARVLECGAMRHTPAGLPALELLLVHESEVVEAGRRVELTISAVALGDLALLLADTPLGTEMQVQGFLAPARK--D-SVKVKLHLQQARRIAGSM--------

General information: TITO was launched using: RESULT: Template: 3FHW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 312 -53585 -171.75 -637.92 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : -171.75 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.273

(partial model without unconserved sides chains): PDB file : Tito_3FHW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FHW-query.scw PDB file : Tito_Scwrl_3FHW.pdb: