Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MEKARISIRQLFVMIIIFELGSSLLITPGSMAGRDAWIAVLLGCAIGLFLFYLYQG-IYQCYPNSSPKEYMDDMLGTKLSWLFSFLYILYFAYIAARVLRDFGEMLLTFAYHD----TPIIIVNALLMV------VSIYAVRKGIEVLARAAELLFGA---MYLLGAIGLVLIIVSGTIDPHNLKPVLANGISPVLHSVFTQTMYVPFGEVVLFVMIFPNLNDRKDVKKMGMIAMAISGLIVALTVAINISVLDVDLTLRSQFPLLSTIQTIKVEEFLDRLDVFFMLALIIGGFFKVSLYLYATVVGTSTLFKEKNPSQLAYPMGLGILILSITIATNFSEHLNEGLNVVPLYIHLPFQLLFPLFLFIVAVWKKKRREKSKGEEAKK 4P6V Chain:B ((38-409)) GLVTKRSSHVRDSVDLKRIMIMVWL------------AVFPAMFWGMYNAGGQAIAALNHLYSGDQLAAIVAGNWHYWLTEMLGGTMGSK-MLLGATYFLPIYATVFIVGGFWEVLFCMVRKHEVNEGFFVTSILFALIVPPTLPLWQAALGITFGVVVAKEVFGGTGRNFLNPALAGRAFLFFAY--PAQISGDLVWTAADGYSGATALSQWAQGGAGAL-------INIPGSIGEVSTLALMIGAAFIVY---MGIASWRIIGGVMIGMILLSTLFNVIGS---DTNAMFNMPWHLVLGGFAFGMFFMAT---DPVSASFTNSGKWAYGILIGVMCVLIRVVNP---AYPEGM----MLAILFANLFAPLFDHVVVERNIKRRL---------

General information: TITO was launched using: RESULT: Template: 4P6V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1519 -258118 -169.93 -791.77 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -169.93 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.158

(partial model without unconserved sides chains): PDB file : Tito_4P6V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4P6V-query.scw PDB file : Tito_Scwrl_4P6V.pdb: