Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDITFNTGLMGSGKSKKLID-DYLIDPK-EKVALSVSLTEDTFSRG--KIESRDGRSLRSINLNRDQFKQNISLLEIIIFMTNTQTIYIDESQFLPKETVEKFVSLSESYHVPIHFYGLDLTFTGELFDSSSHLLTILPSENINRISRGCEASKCSKIAQYNARIVDGKVSRSGETFVEEKSY-YLALCSDHYYNDEKII 1W4R Chain:A ((20-192)) GQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIK---YAKDTRYSSSFCTHDRNTMEALPAC--------LLRDVAQEALGVAVIGIDEGQFFPD-IVEFCEAMAN-AGKTVIVAALDGTFQRKPFGAILNLVP--LAESVVKLTAVCME--CFREAAYTKRLGTE-----KEVEVIGGADKYHSVCRLCYFK-----

General information: TITO was launched using: RESULT: Template: 1W4R.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 898 -91193 -101.55 -542.81 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -101.55 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.330

(partial model without unconserved sides chains): PDB file : Tito_1W4R.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W4R-query.scw PDB file : Tito_Scwrl_1W4R.pdb: