Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MADNNKMSREEAG-RKGGETTSKNHDKEFYQEIGQKGGEA-TSKNHDKEFYQEIGEKGGEATSKNHDKEFYQEIGEKGGEATS---ENHDKEFYQEIGRKGGEATSKNHDKEFYQEIGSKGGNARNND 2JN9 Chain:A ((1-105)) MVKTLRLNNVTLEMAAYQEESEPKRKIAFTLNVTSETYHDIAVLLYEKTFNVEVPERD---------LAFRGEMTNYSTSLTNLYEPGAVSEFYIEITEIDKNADSLEHHHHHH--------------

General information: TITO was launched using: RESULT: Template: 2JN9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 369 13473 36.51 134.73 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 36.51 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.138

(partial model without unconserved sides chains): PDB file : Tito_2JN9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JN9-query.scw PDB file : Tito_Scwrl_2JN9.pdb: