Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFIRTENFQTFIRLYPVVTFILALQAVLWLFFSLPAHSVVLWRDTVTGYNLGVANGEWWRLITPILLHAGFTHLLFNSMSIFLFAPALERMLGKARFLLVYAGSGIIGNIGTYVTEPLDYVHVGA-SGAIFGLFGVYLFMVLFRNELIGQEHSKMIITLLAFAVL-----------MSFINSNINMMAHLFGLCGGFLLSFLCVQKKERRY 3ZMH Chain:A ((44-177)) --------------------------------------------------------EFWRYFTHALMHFSLMHILFNLLWWWYLGGAVEKRLGSGKLIVITLISALLSG---YVQQKFSGPWFGGLSGVVYALMGY----VWLRGERDPQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANG-----AHIAGLAVGLAMAFV---------

General information: TITO was launched using: RESULT: Template: 3ZMH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 517 -85026 -164.46 -696.93 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -164.46 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.267

(partial model without unconserved sides chains): PDB file : Tito_3ZMH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZMH-query.scw PDB file : Tito_Scwrl_3ZMH.pdb: