Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------------------------------------------------------------------------------------------------------------------MNHIKWLSDLKKAGLLALGKGV--ITLLKRFSLVIVTLMMSIVVVLAAAKESPGDHVISFDEPIILMISIGIVVFLLIPPLVMSFFGNLVVRIISGVYQCFIVFTFLGLIPIGFLIPNGFLTILVSIAGTLVSIASVAVTLCIGKNKKDI----------------------------------------------------------------------------------------------------------------------------------------------------------------- 2EUA Chain:A ((2-429)) QSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNGDEEAVVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTTTREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLN--------CYHFYMAFDGE------------------------------NAFLGSSP----------ERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLMADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQ

General information: TITO was launched using: RESULT: Template: 2EUA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 157 -30010 -191.14 -300.10 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -191.14 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.53 QMean score : -0.005

(partial model without unconserved sides chains): PDB file : Tito_2EUA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EUA-query.scw PDB file : Tito_Scwrl_2EUA.pdb: