Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQRVSYYEIAPEGMKIMMDMEKY-TK-QSSINRTTRELIKIRVSQMNGCAFCIDMHTSDARKMGETEQRIYCLHAWNECDFYSPEEKAALELSEHITLIPSKRVPDELYHRVREH----YDEEQYVDLVLIINQINSWNRISIAMGNRAASK 5DJ4 Chain:A ((78-269)) --NLAVVMGLHPDYFTSFWRLHYLLLHTDGPLASSWRHYIAIMAAARHQCSYLVGSHMAEFLQTGGDPEWLLGLH------RAPEKLRKLSEINKLLAHRP-WLITKEHIQALLKTGEHTWSLAELIQALVLLTHCHSLSSFVFGCGILPEG-

General information: TITO was launched using: RESULT: Template: 5DJ4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 492 -76333 -155.15 -557.17 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -155.15 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.338

(partial model without unconserved sides chains): PDB file : Tito_5DJ4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DJ4-query.scw PDB file : Tito_Scwrl_5DJ4.pdb: