Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------MEDAGLLLIRIM-IGVVFLF-YGTQKLFGWFGGYGIKGTG--------QWFESIG-V-K-PGNVAAALSGLGELVSGILFILGVFLPLGAAIITIIMLGAIVKVHGAKGFANGAGGFEYNLVLIA-----VSIGVALIGSGAYALHF-------------------------------------- 3GW4 Chain:A ((8-199)) DYALAERQAQALLAHPATASGARFMLGYVYAFMDRFDEARASFQALQQQAQKSGDHTAEHRALHQVGMVERMAGNWDAARRCFLEERELLASLP----EDPLAASANAYEVATVALHFGD-LAGARQEYEKSLVYAQQADDQVAIACAFRGLGDLAQQEKNLLEAQQHWLRARDIFAELEDSEAVNELMTRLNGLEH

General information: TITO was launched using: RESULT: Template: 3GW4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 448 -47060 -105.04 -379.51 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -105.04 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_3GW4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GW4-query.scw PDB file : Tito_Scwrl_3GW4.pdb: