Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDFAFKLYEYNVWANQQIFNRLKELPKEIYHQEIQSVFPSISHVLSHVYLSDLGWIE-VFSGKTL--SDALALA-----E-QLKEQTEAKEIEEMEDLFLRLSERYILFLQQ--KEQLNKPLQIQNPSSGIMKTTVSELLPHVVNHGTYHRGNITAMLRQAGYASAPTDYGLYLFMTKTEKA 2OU6 Chain:A ((30-185)) FTPHIGALVEMLTYARLTTLQAVEGLPEDQLWATAPGFANSIGTLLAHIAAVERVYHVLSFQGRDVTPEDDGAAYWGLTMGKEGTAPARLPTLDELRAELADARAETLRVFAAKDDAWLAEPLG----P---GWANQHWAWFHVMEDEVNHRGQLRLLRQVLA--------------------

General information: TITO was launched using: RESULT: Template: 2OU6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 550 -64171 -116.67 -442.56 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -116.67 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.506

(partial model without unconserved sides chains): PDB file : Tito_2OU6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OU6-query.scw PDB file : Tito_Scwrl_2OU6.pdb: