Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHPLFLEPVFKERLWGGT-KLRDAFGYAIP-SQKTGECWAVSAHAHGSSSVKNGPLAGKTLDQVWKDHPE-IFGFPD---GKVFPLLVKLLDANMDLSVQVHPDDDYAKLHEN-----------------GDLGKTECWYIIDC-------------------KDDAELILGHHA-S-TK----------------------EEFKQRIESGD---W------------------NGLLRRIKIKPGDFFYVPSGTLHALCKGTLVLEIQQNSDTTYRVYDYDRCNDQGQKRTLHIEKAMEVITIPHIDKVHTPEVKEVGNAEIIV--YVQSDYFSVYKWKISGRAAFPSYQTYLLGSVLSGSGRIINNGIQYECNAGSHFILPAHFGEFTIEGTCEFMISHP 2WFP Chain:A ((1-391)) MASMQKLINSVQNYAWGSKTALTELYGIANPQQQPMAELWM-GAHPKSSSRIT-----TVSLRDAIEKNKTAMLGEAVANRFGELPFLFKVLCAAQPLSIQVHPNKRNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSDIVSLLQPVAGAHSAIAHFLQVPNAERLSQLFASLLNMQGEEKSRALAVLKAALNSQQGEPWQTIRVISEYYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGV-ALEVMANSDNVLRA------GLT--PKYIDIPELVANVKFEPKPAGELLTAPVKSGAELDFPIPVDDFAFSLHDLALQET--SIGQHSAAILFCVEGEAVLRKDEQRLVLKPGESAFIGADESPVNASGTGRLARVY-

General information: TITO was launched using: RESULT: Template: 2WFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1546 -133139 -86.12 -448.28 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -86.12 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.370

(partial model without unconserved sides chains): PDB file : Tito_2WFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WFP-query.scw PDB file : Tito_Scwrl_2WFP.pdb: