Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNETITYDTWNDMLSKQITDQLIDELDVLKWAYRTYGEKIVYACSFGAEGMVLLDLISKINKNAHIIFLDTGLHFQETYELIETVKERYPGFAIQMLEPELSLTEQGTKYGG--ELWKHNPNLCCQLRKIEPLKKHLSGMTAWISGLRRDQSP-TRKHIQYVNLDQKFE-----LIKICPLIHWTWDDVWTYIRLHNLPYNKLHDQHYPSIGCEMCTLPSPDPNDERAGRWAGREKTECGLHQE 2GOY Chain:G ((44-249)) --------------------------DILKAAFEHFGDELWISFS-GAEDVVLVDMAWKLNRNVKVFSLDTGRLHPETYRFIDQVREHY-GIAIDVLSPDPRLLEPLVKEKGLFSFYRDGHGECCGIRKIEPLKRKLAGVRAWATGQRRDQSPGTRSQVAVLEIDGAFSTPEKPLYKFNPLSSMTSEEVWGYIRMLELPYNSLHERGYISIGCEPCTRPVLPNQHEREGRWWWE----------

General information: TITO was launched using: RESULT: Template: 2GOY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 929 -102200 -110.01 -516.16 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain G : 0.79 3D Compatibility (PKB) : -110.01 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.371

(partial model without unconserved sides chains): PDB file : Tito_2GOY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GOY-query.scw PDB file : Tito_Scwrl_2GOY.pdb: