Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATLSDVAKKANVSKMTVSRVINHPETVTDELKKLVHSAMKELNYIPNYAARALVQNRTQVVKLLILEEMDTTEPYYMNLLTGISRELDRNHYALQL--------VTRNSLNI---GQCDGIIATGLRKNDFEGVIKAFEK----PLVVFGQNEMGYDFIDV---NNEKGTFMATRHVMGLGEREVVFFGIDLDEPFER----AREQGYIRAMNKSFKK---SNMFRIDNSSKKSECLARELLKSMDNQAAFVCASDRIALGVIRAAQSLGKRIPEDVAVTGNDGVFLDRISSPRLTTVRQPVVEMGEACAKMLLKKMNEDGAAQGSLFFEPELIVRESTL 1JFT Chain:A ((1-329)) -ATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELHYSPSAVARSLKVNHTKSIGLL---ATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLEKQRAYLSMMAQKRVDGLLV--MCSEYPEPLLAMLEEYRHIPMVVMDAGEAKADFTDAVIDNAFEGGYMAGRYLIERGHREI---GV-IPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQGDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDNVRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRS--

General information: TITO was launched using: RESULT: Template: 1JFT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1561 -18654 -11.95 -61.36 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -11.95 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_1JFT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JFT-query.scw PDB file : Tito_Scwrl_1JFT.pdb: