Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------MNY-EIFKAIHGLSHH-NSVLDSIMVFITEYA------IVAYALILLAIWLFGNTQSRKHVLYAGITG--IAGLVINYLITLVYFEPRPFVAHTVHT-----------------------------LIPHAADAS---FPSDHT-TGA--LAISIAMLFRNRKIGWPLVIFGLLTGF--SRIWVG-HHYPVDVLGSLVVAIIIGFLFFRFSDLLRPFVDLVVRIYEAIINKLTKKPTDQNF 4PX7 Chain:A ((1-258)) GRSIARRTAVGAALLLVMPVAVWISGWRWQPGSWLLKAAFWVTETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQEPRPFVIWLEKTHHMPVDKFYTLKRAERGNLVKEQLAEPQYLRSHWQKETGFAFPSGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLISWALVAVATWLAQRICGPLTPPE----ENREIAQREQESLEHH

General information: TITO was launched using: RESULT: Template: 4PX7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 481 -40067 -83.30 -211.99 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -83.30 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.086

(partial model without unconserved sides chains): PDB file : Tito_4PX7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PX7-query.scw PDB file : Tito_Scwrl_4PX7.pdb: