Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKSAIFVLSGFLAFLLYALLLYGLLLERHNKEAEKILLDLNKKDEQAIDLNLEDLPSEKKNEKIEKVTEKQGDFLEPKEEPKEEPEESLEDIFS-----SLNDFQEKTDKNAQKDEQKNEQEEQRRLREQQRLKQNQENQEMLKGLQQNLNQFTQKLESVKNKTLDLQIPKQDGVDEKAYQEWYAQIYQILYKGWRGVFYHKASVSVLIMITKDGEFDYTILSYSDFKDYNKSVMTLLDDLKKVDFPPYPGGNMISIKVNFTTKEEQ 3TNF Chain:B ((7-111)) ---------------------------------------------EQAIKRAQENIKKGEELEKKLDKLERQGKDLEDKYKTYEENLEGFEKLLTDSEELSLSEINEKMEAFS-KDSEKLTQLMEKHKGDEKTVQSLQREHHDIKAKLANLQVLHDAHTGKKSYVNEKGNPVSSLKDAHLAINKDQEVVEHEGQFYLLQKGQWDAIKNNPAALEKAQKDYSQSKHDLATIKMEALIHKLSLEMEKQLETINNLIMSTDPKENEEATKL

General information: TITO was launched using: RESULT: Template: 3TNF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) 43262 for 461 contacts (93.8/contact) + 2D Compatibility (PS) -10943 + (NN) -1621 + (LL) 4408 1D Compatibility (HY) -1200 + (ID) 1450 Total energy: 32456.0 ( 70.40 by residue) QMean score : 0.364

(partial model without unconserved sides chains): PDB file : Tito_3TNF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TNF-query.scw PDB file : Tito_Scwrl_3TNF.pdb: