Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRAEEQSKIREAAAGTIFLLIGTVCFASKSIWIKWAYQMGAEPDAVLLYRQLLAVPLFWL--IFLIYRPPMPDGKKKGDLWKACGAGVFCFFLSPLLDFIGLNHVSAMVERILLMSYPLFVFGFTACRDRKMSSIQDLFAVLAVMFGLFLALGGWNAELFQANMIGAVFILLSSAVYAGYLVLSGHLVHQIGGIRLNAYGMTAAGAAMMLYTGIKSAAGMNTPMAAYPLSMYGLFAVIAVVTTVIPFVLMLEGIKRIGAQRAAAISMAGPILTIFYGALFLGERLGLIQVIGCGGVFFVITGMEYRKLKTGKKE 2MTP Chain:B ((988-1008)) ----------------------------------------------------------WKVGFFKRNRPPLEEDDEEGE-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2MTP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 13 1123 86.35 59.08 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.38 3D Compatibility (PKB) : 86.35 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.38 QMean score : 0.321

(partial model without unconserved sides chains): PDB file : Tito_2MTP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MTP-query.scw PDB file : Tito_Scwrl_2MTP.pdb: