Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITLFQCLYLILFSFICYQGAAAFSHSTAASWLAAALGAAAAGLYIWNTKRVWKHCSSGLCAWIAVIQVMSVGVVLIGTDIMPVLCVIAIFAGCEGLRIGQSALQARLSDQIDKLTQAEQHANQMLIDVRSRNHDTMKHITAIKSA-Q---P----KADTQAYIQNWADQYSQYDRFLKGE---------------NAYVAGVLYDFLEKARAS--NVSVSLHMHTPLSSLPFSPADQVSLVGNILENALDSAAEAREKAEIKLETSLRSGLYVLTCENSTPGMDPKVLDTIYQSFGRSTKN--GAHEGMGTYIIQKLVKGAFGRLDFTYRHP-IFRLEIKIPFQK 4I5S Chain:A ((208-432)) ----------------------------------------------------------------------------------------------------------------------------RRLFVSNVSHELRTPLTSVKSYLEALDDGALTESVAPSFIKVSLDETNRMMRMITDLLSLSRSHLDVELTNFTAFMNYILDRFDQIQSQQST--EIIRDYPDKSVWIEIDTDKMTQVIDNILNNAIKYSPDG---GKVTITMQTTDTQLILSISDQGLGIPKKDLPLIFD-RFYRVDKAR----GLGLAIAKEIVKQHKGFIWANSEEGEGSTFTIVLP---

General information: TITO was launched using: RESULT: Template: 4I5S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 805 -62206 -77.27 -343.68 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -77.27 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.341

(partial model without unconserved sides chains): PDB file : Tito_4I5S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4I5S-query.scw PDB file : Tito_Scwrl_4I5S.pdb: