Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEVIKSLTDHRSIRSYTDEPVAQEQLDQIIEAVQSAPSSINGQQVTVITVQDKERKKKISELAG-----------GQPWI---------------DQAPVFLLFCADFNRAKIALEDLHDFKMEITNGLESV-LVGAVDAGIALGTATAAAESLGLGTVPIGAVRGNPQELIELLELPKYVFPLSGLVIGHPADRSAKKPRLPQEAVNHQETYLNQDELTSHIQAYDEQMSEYMNKRTNGKETRNWSQSIASYYERLYYPHIREMLEKQGFKVEK 4TTC Chain:A ((62-253)) --EFYELLNKRRSVRFISNEQVPMEVIDNVIRTAGTAPSGAHTEPWTFVVVKDPDVKHKIRKIIEEEEEINYMKRMGHRWVTDLKKLRTNWIKEYLDTAPILILI----------FKQVHGFA---ANGKKKVHYYNEISVSIACGILLAALQNAGLVTVTTTPLNCGPR-LRVLLGRPAHEKLLMLLPVGYPS-KEATVPDLKRKPLD-------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4TTC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 659 -98837 -149.98 -599.01 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -149.98 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.608

(partial model without unconserved sides chains): PDB file : Tito_4TTC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TTC-query.scw PDB file : Tito_Scwrl_4TTC.pdb: